Chain-melting phase transition in a lamellar film of dimyristoyl-phosphatidylserine on the surface of a silica hydrosol

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Abstract

Structural dynamics of multilayer of dimyristoyl-phosphatidylserine formed on the surface of silica sol with 5 nm nanoparticles size has been investigated by X-ray reflectometry and grazing-incidence diffraction at 71 keV photon energy. Combined model-based and modelless analysis of reflectometry data revealed the structure consisting of a surface monolayer and a stack of lamellar bilayers sandwiched between water layers, with a spatial period of ~ 150 Å. With increase in temperature above the chain-melting point the surface monolayer is observed to transition from a surface crystal phase with minimal area-per-lipid value of (40 ± 1) Å2 to a disordered liquid phase with estimated area-per-lipid value of (52 ± 2) Å2. Under low temperatures both monolayer and bilayer slabs contain 5 to 8 H2O molecules bound to lipid PS-fragment; however, above the melting point the amount of contained water rises to about 14 molecules per bilayer headgroup.

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About the authors

A. M. Tikhonov

P.L. Kapitza Institute for Physical Problems Russian Academy of Sciences

Author for correspondence.
Email: tikhonov@kapitza.ras.ru
Russian Federation, Moscow

Yu. O. Volkov

Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics of NRC “Kurchatov Institute”

Email: tikhonov@kapitza.ras.ru
Russian Federation, Moscow

A. D. Nuzhdin

Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics of NRC “Kurchatov Institute”

Email: tikhonov@kapitza.ras.ru
Russian Federation, Moscow

B. S. Roshchin

Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics of NRC “Kurchatov Institute”

Email: tikhonov@kapitza.ras.ru
Russian Federation, Moscow

V. E. Asadchikov

Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics of NRC “Kurchatov Institute”

Email: tikhonov@kapitza.ras.ru
Russian Federation, Moscow

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2. Fig. 1. X-ray reflectivity curves R(qz) for a DMPS multilayer on a silica hydrosol surface with 5 nm nanoparticles. Curves 1–5 correspond to data obtained at T ≈ 23, 28, 34, 37, and 40°C, respectively. Solid and dashed lines correspond to the results of model-free and model reconstruction, respectively. Inset: X-ray scattering kinematics at the air–sol interface.

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3. Fig. 2. Integrated intensity of grazing diffraction ID(q||) from DMPS multilayer. Circles and squares represent data obtained at 23 (1) and 40°C (2), respectively. The solid line illustrates the approximation of the diffraction peak by the Gaussian function.

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4. Fig. 3. Electron density profiles ρ(z) calculated within the model-independent (solid lines) and model (dashed lines) approaches. Curves 1–5 correspond to data obtained at T ≈ 23, 28, 34, 37, and 40°C, respectively. For convenience, the curves are shifted along the ordinate axis. The values ​​are normalized to the electron density of water under normal conditions ρw = 0.333 e/Å–3.

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5. Fig. 4. Chemical structure of the ionized DMPS molecule. Hydrophobic components are shown as dotted lines.

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6. Fig. 5. Structural model of a multilayer DMPS film constructed based on the analysis of model-free calculations.

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