Chain-melting phase transition in a lamellar film of dimyristoyl-phosphatidylserine on the surface of a silica hydrosol

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Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Тек жазылушылар үшін

Аннотация

Structural dynamics of multilayer of dimyristoyl-phosphatidylserine formed on the surface of silica sol with 5 nm nanoparticles size has been investigated by X-ray reflectometry and grazing-incidence diffraction at 71 keV photon energy. Combined model-based and modelless analysis of reflectometry data revealed the structure consisting of a surface monolayer and a stack of lamellar bilayers sandwiched between water layers, with a spatial period of ~ 150 Å. With increase in temperature above the chain-melting point the surface monolayer is observed to transition from a surface crystal phase with minimal area-per-lipid value of (40 ± 1) Å2 to a disordered liquid phase with estimated area-per-lipid value of (52 ± 2) Å2. Under low temperatures both monolayer and bilayer slabs contain 5 to 8 H2O molecules bound to lipid PS-fragment; however, above the melting point the amount of contained water rises to about 14 molecules per bilayer headgroup.

Толық мәтін

Рұқсат жабық

Авторлар туралы

A. Tikhonov

P.L. Kapitza Institute for Physical Problems Russian Academy of Sciences

Хат алмасуға жауапты Автор.
Email: tikhonov@kapitza.ras.ru
Ресей, Moscow

Yu. Volkov

Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics of NRC “Kurchatov Institute”

Email: tikhonov@kapitza.ras.ru
Ресей, Moscow

A. Nuzhdin

Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics of NRC “Kurchatov Institute”

Email: tikhonov@kapitza.ras.ru
Ресей, Moscow

B. Roshchin

Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics of NRC “Kurchatov Institute”

Email: tikhonov@kapitza.ras.ru
Ресей, Moscow

V. Asadchikov

Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics of NRC “Kurchatov Institute”

Email: tikhonov@kapitza.ras.ru
Ресей, Moscow

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2. Fig. 1. X-ray reflectivity curves R(qz) for a DMPS multilayer on a silica hydrosol surface with 5 nm nanoparticles. Curves 1–5 correspond to data obtained at T ≈ 23, 28, 34, 37, and 40°C, respectively. Solid and dashed lines correspond to the results of model-free and model reconstruction, respectively. Inset: X-ray scattering kinematics at the air–sol interface.

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3. Fig. 2. Integrated intensity of grazing diffraction ID(q||) from DMPS multilayer. Circles and squares represent data obtained at 23 (1) and 40°C (2), respectively. The solid line illustrates the approximation of the diffraction peak by the Gaussian function.

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4. Fig. 3. Electron density profiles ρ(z) calculated within the model-independent (solid lines) and model (dashed lines) approaches. Curves 1–5 correspond to data obtained at T ≈ 23, 28, 34, 37, and 40°C, respectively. For convenience, the curves are shifted along the ordinate axis. The values ​​are normalized to the electron density of water under normal conditions ρw = 0.333 e/Å–3.

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5. Fig. 4. Chemical structure of the ionized DMPS molecule. Hydrophobic components are shown as dotted lines.

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6. Fig. 5. Structural model of a multilayer DMPS film constructed based on the analysis of model-free calculations.

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