Email this article Phenomenon of hyperexpression of rybosomiccistrons in bone marrow hemopoietic elements in bronchialasthma
- Authors: Mineev VN1, Mamaev NN1, Ivanovа VV1, Nesterovich II1, Gindina TL1
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Affiliations:
- Issue: Vol 79, No 12 (2004)
- Pages: 53-58
- Section: Editorial
- URL: https://ter-arkhiv.ru/0040-3660/article/view/29962
- ID: 29962
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Abstract
Aim. To study expression of ribosomic cistrons (RC) of bone marrow hemopoietic elements (BMHE) in
bronchial asthma (BA).
Material and methods. A total of 37 bronchial asthma (BA) patients were examined. Of them, 20
ones had allergic BA (ABA) and 10 - nonallergic BA (NABA). Seven patients received systemic glucocorticoid
hormones (SGH). Control included cells of bone marrow from 7 healthy donors. Transcription
and maturation of pre-rRNA in BMHE were analysed using bone marrow samples obtained
during the sternal puncture (a modified method of Howell and Black).
Results. RC activity was high. It depended on BA variant and the disease phase. In ABA high RC activity
persisted in remission. GCH suppress RC activity rise. The maximal effect of GCH suppressive
action was seen in myeloid lineage, minimal - in erythroid one. The effect is absent in more mature
erythroid cells and bone marrow lymphocytes.
Conclusion. The phenomenon of RC activity rise in myeloid, erythroid and lymphoid lineages of the
bone marrow of BA patients may reflect contribution of the bone marrow to formation of BA pathogenesis.
bronchial asthma (BA).
Material and methods. A total of 37 bronchial asthma (BA) patients were examined. Of them, 20
ones had allergic BA (ABA) and 10 - nonallergic BA (NABA). Seven patients received systemic glucocorticoid
hormones (SGH). Control included cells of bone marrow from 7 healthy donors. Transcription
and maturation of pre-rRNA in BMHE were analysed using bone marrow samples obtained
during the sternal puncture (a modified method of Howell and Black).
Results. RC activity was high. It depended on BA variant and the disease phase. In ABA high RC activity
persisted in remission. GCH suppress RC activity rise. The maximal effect of GCH suppressive
action was seen in myeloid lineage, minimal - in erythroid one. The effect is absent in more mature
erythroid cells and bone marrow lymphocytes.
Conclusion. The phenomenon of RC activity rise in myeloid, erythroid and lymphoid lineages of the
bone marrow of BA patients may reflect contribution of the bone marrow to formation of BA pathogenesis.
References
- Denburg J. A., Inman M. D., Leber В. et al. The role of the bone marrow in allergy and asthma. Allergy 1996; 51(3): 141 - 148.
- Denburg J. A., Sehmi R., Upham J. et al. Regulation of IL-5 and IL-5 receptor expression in the bone marrow of allergic asthmatics. Int. Arch. Allergy Immunol. 1999; 118(2-4): 101-103.
- Минеев В. H., Иванова В. В. Роль костного мозга в патогенезе аллергических заболеваний. В кн.: Федосеева Г. Б. (ред.) Общая аллергология. СПб.: Нордмед-Издат; 2001; 1: 517-530.
- Чучалин А. Г. (ред.) Глобальная стратегия лечения и профилактики бронхиальной астмы. М.: Изд-во "Атмосфера"; 2002.
- Howell W. M., Black D. A. Controlled silver-staining of nucleolus organizer with a protective colloidal developer: a one-step method. Experientia 1980; 36: 1014-1015.
- Мамаев Н. H. Использование метода серебрения цистронов рибосомной РНК интерфазных клеток для количественной оценки гемопоэза при заболеваниях крови: (Метод. рекомендации). СПб.; 1998.
- Гланц М., Стентон А. Медико-биологическая статистика: Пер. с англ. Ю. А. Данилова; под ред. Н. Е. Бузикашвили,Д. В. Самойлова. М.: Практика; 1999.
- Simon H. U. Molecular mechanisms of defective eosinophil apoptosis in diseases associated with eosinophilia. Int. Arch. Allergy Immunol. 1997; 113: 206-208.
- Салогуб Г. H. Активность ядрышковых организаторов интерфазных клеток костного мозга больных лейкозами (клинико-морфологические сопоставления): Автореф. дис. ... канд. мед. наук. СПб.; 1997.
- Cheng К. F., Barnes P. J. Cytokines in asthma. Review. Thorax 1999; 54: 825-857.
- Agosti J. M., Sprenger J. D., Lum L. G. et al. Transfer of allergen-specific IgE-mediated hypersensitivity with allogeneic bone marrow transplantation. N. Engl. J. Med. 1988; 319: 1623-1628.
- Higenbottam Т., Varma N. Asthma: an inherited of bone marrow cells? Eur. Respir. J. 1989; 2(10): 921-922.
- Walker S. A., Riches P. G., mid G. et al. Total and allergenspecific IgE in relation to allergic response pattern following bone marrow transplantation. Clin. Exp. Immunol. 1986; 66(3): 633-639.
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