Density-specific distribution of erythrocytes in different types of anemia

Abstract

Aim. To study density-specific distribution of erythrocytes (DSDE) in different types of anemia.
Material and methods. DSDE was determined in anemic patients by fractionation of the whole blood in hematocritic capillaries in the presence of mixtures of dimethyl- and dibutylphthalates with known density.
Results. Parameters are proposed which characterize DSDE changes typical for each type of anemia: mean erythrocyte density (MED) - mean density of total erythrocytic population; DSDE width (W) - a characteristic of erythrocytic population heterogeneity; light fraction of erythrocytes (LEF) - % of the cells with density less than 1.086 g/ml (hypochromic cells and reticulocytes); dense fraction of erythrocytes (DEF) - % of cells with density over 1.112 g/ml (hyperchromic cells forming as a result of erythrocyte dehydration). DSDE parameters for different types of anemia differed: reduced MED was typical for iron deficiency anemia (IDA) and paroxysmal nocturnal hemoglobinuria (PNH), increased DEF was seen in microspherocytic anemia (MSA), autoimmune hemolytic anemia (AHA), deficiency of glucose-6-phosphate dehydrogenase, increased LEF was observed in reticulocytosis in all anemia types except MSA, DSDE W was larger in MSA, AHA, PNA.
Conclusion. DSDE is determined by proportion of erythropoiesis and sequestration of erythrocytes as well as pathological impacts leading to impairment of membrane permeability for cations and erythrocytic metabolism. Informative value of DSDE parameters makes them effective for diagnostic screening of anemias and control over course of different diseases.

References

  1. Lutz H. U., Red cell density and red cell age. Blood Cells 1988; 14: 76-80.
  2. Nakashima K., Oda S., Miwa S. Red cell density in various blood disorders. J. Lab. Clin. Med. 1973; 82: 297-302.
  3. Шурхина Е. С., Цветаева Н. В., Колодей С. В. и др. Плотность и деформируемость эритроцитов больного АИГА с антифосфолипидным синдромом в разные периоды течения болезни. Возможность прогнозирования обострения. Гемтаол. и трансфузиол. 2003; 48 (3): 19-22.
  4. Шурхина Е. С., Нестеренко В. М., Лисовская И. Л. и др. Выявление этапов аутоиммунной гемолитической анемии с помощью исследования деформируемости и плотности эритроцитов. Бюл. экспер. биол. 2004; 138 (9): 316-319.
  5. Danon D., Marikovasky Y. Determination of density distribution of red cell population. J. Lab. Clin. Med. 1964; 64: 668-673.
  6. Сторожок С. А., Санников А. Г., Захаров Ю. М. Молекулярная структура мембран эритроцитов и их механические свойства. Тюмень; 1997. 76-104.
  7. Feig S. A., Bassilian S. Increased erythrocyte C2+ content in hereditary spherocytosis. Pediatr. Res. 1975; 9 (12): 928-931.
  8. Da Costa L., Mohandas N., Sorette M. et al. Temporal differences in membrane loss lead to distinct reticulocyte features in hereditary spherocytosis and in immune hemolytic anemia. Blood 2001; 98 (10): 2894-2899.
  9. Muller-Eberhand H. J. The membrane attack complex of complement. Annu. Rev. Immunol. 1986; 4: 503-528.
  10. Halperin J. A., Brugnara C., Nicholson-Weller A. Ca2+-activated K+ efflux limits complement mediated lysis. J. Clin. Invest. 1989; 83: 1466-1471.
  11. Iida K., Whitlow M. B., Nussenzweig V. Membrane vesiculation protects erythrocytes from destruction by complement. J. Immunol. 1991; 147 (8): 2638-2642.
  12. Pascual M., Lutz H. U., Steiger G. et al. Release of vesicles enriched in complement receptor 1 from human erythrocytes. J. Imunol. 1993; 51: 397-404.
  13. Hall S. E., Rosse W. F. The use of monoclonal antibodies in the diagnosis of paroxysmal nocturnal hemoglobinyria. Blood 1996; 87 (12): 5332-5340.
  14. Beppu M., Mizukami A., Nagoya M., Kikugawa K., Binding of anti-band 3 antibody to oxidatively damaged erythrocytes. J. Biol. Chem. 1990; 265: 3226-3229.
  15. Turrini F., Naitana A., Mannuzzu L. et al. Increased red cell calcium, decreased calcium adenosine triphosphatase, and altered membrane proteins during fava bean hemolysis in glucose-6-phosphate dehydrogenase-deficient (Mediterranean variant) individuals. Blood 1985; 66 (2): 302-305.

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Copyright (c) 2009 Shukhrina E.S., Nesterenko V.M., Kolodey S.V., Tsvetaeva N.V., Ermakova T.A., Nikulina O.F., Kolosheynova T.I., Ataullakhanov F.I., Shukhrina E.S., Nesterenko V.M., Kolodey S.V., Tsvetaeva N.V., Ermakova T.A., Nikulina O.F., Kolosheinova T.I., Ataullakhanov F.I.

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