Determination of subepithelial deposits of IgA antibodies to tissue transglutaminase: practical experience with application for diagnosis of gluten-sensitive celiac disease


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Abstract

Aim. To estimate subepithelial deposites of IgA antibodies to tissue transglutaminase (IGA-tTG) in biopsy specimens of small intestinal mucosa and to determine its diagnostic significance for detection of gluten-sensitive celiac disease (GSCD).
Material and methods. Enzyme immunoassay examined IGA-tTG deposits after their isolation from biopsy specimens of small intestinal mucosa of 53 patients with GSCD and 45 control patients.
Results. Elevated levels of IGA-tTG deposits were found in 98% patients with GSCD. This elevation in the controls occurred in patients with autoimmune pathology and in patients with such levels of the serum markers which were in the range of reference limits in the absence of GSCD morphometric characteristics in biopsy specimen but with verified diagnosis celiac disease.
Conclusion. This method showed high diagnostic efficacy in detection of subepithelial IGA-tTG deposits for diagnosis of GSCD and monitoring of pathogenetic diet therapy.

About the authors

Natal'ya Vasil'evna Vokhmyanina

Email: spbnat@yandex.ru

A V Kozlov

Asfol'd Ivanovich Parfenov

Email: asfold@mail.ru

Lyudmila Savarbekovna Oreshko

Email: oreshko@yandex.ru

N V Vokhmyanina

Medicogenetic Diagnostic Center, Moscow

Medicogenetic Diagnostic Center, Moscow

A V Kozlov

Medical Academy of Postgraduate Education, St-Peterburg

Medical Academy of Postgraduate Education, St-Peterburg

A I Parfenov

Central Research Institute of Gastroenterology, Moscow

Central Research Institute of Gastroenterology, Moscow

L S Oreshko

I.I. Mechnikov St-Petersburg State Medical Academy

I.I. Mechnikov St-Petersburg State Medical Academy

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Copyright (c) 2012 Vokhmyanina N.V., Kozlov A.V., Parfenov A.I., Oreshko L.S., Vokhmyanina N.V., Kozlov A.V., Parfenov A.I., Oreshko L.S.

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