Email this article Computer morphometry of lymphocytes in the diagnosis of chronic transplant nephropathy
- Authors: Vatazin AV1, Valov AL2, Vasilenko IA3, Shcherbakova EO1, Metelin VB3
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Affiliations:
- M.F. Vladimirsky Moscow Regional Research Clinical Institute
- Russian Children's Clinical Hospital, Russian Agency for Health Care
- Russian Gerontology Research Clinical Center, Russian Agency for Health Care
- Issue: Vol 82, No 6 (2010)
- Pages: 46-49
- Section: Editorial
- URL: https://ter-arkhiv.ru/0040-3660/article/view/30621
- ID: 30621
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Abstract
Subjects and methods. Thirty-seven renal graft recipients (29 males and 8 females; mean age 35.9±2.2 years; mean time after renal allografting 19.9±2.7 months). Twenty-three patients had satisfactory renal graft function; 14 patients were diagnosed as having CTN. A control group included 30 apparently healthy individuals. Along with physical examination, vital computer phase morphometry (CPM) of peripheral blood immunocompetent cells was carried out in all the patients.
Results. Small and average cells were predominant in the T and B lymphocyte populations. In CTN, there were increases in diameter, perimeter, and area and decreases in the phase height and volume of B lymphocytes, which is indicative of their activation. There was a predominance of average B lymphocytes in CTN.
Conclusion. Vital CPM of peripheral blood immunocompetent cells may be used for the early diagnosis and prediction of CTN development.
About the authors
A V Vatazin
M.F. Vladimirsky Moscow Regional Research Clinical Institute
Email: svia@rambler.ru, hadik2@rol.ru
M.F. Vladimirsky Moscow Regional Research Clinical Institute
A L Valov
Russian Children's Clinical Hospital, Russian Agency for Health Careотделение по пересадке почкиканд. мед. наук, зав; ГУ Российская детская клиническая больница Росздрава; Russian Children's Clinical Hospital, Russian Agency for Health Care
I A Vasilenko
Russian Gerontology Research Clinical Center, Russian Agency for Health Care
Email: svia@rambler.ru, hadik2@rol.ru
лаборатория компьютерной цитоморфометриид-р мед. наук, проф., зав; ФГУ Российский геронтологический научно-клинический центр Росздрава; Russian Gerontology Research Clinical Center, Russian Agency for Health Care
E O Shcherbakova
M.F. Vladimirsky Moscow Regional Research Clinical InstituteM.F. Vladimirsky Moscow Regional Research Clinical Institute
V B Metelin
Russian Gerontology Research Clinical Center, Russian Agency for Health Careлаборатория компьютерной цитоморфометрииканд. биол. наук, ст. науч. сотр; ФГУ Российский геронтологический научно-клинический центр Росздрава; Russian Gerontology Research Clinical Center, Russian Agency for Health Care
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