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Structure of the carboxypeptidase t from Thermoactinomyces vulgaris complex with L - phenyl lactate
- Authors: Akparov V.K.1, Konstantinova G.E.1, Timofeev V.I.1, Shvetsov M.B.2, Kuranova I.P.1,2
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Affiliations:
- Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics of NRC “Kurchatov Institute”
- Moscow Institute of Physics and Technology
- Issue: Vol 69, No 3 (2024)
- Pages: 422-428
- Section: STRUCTURE OF MACROMOLECULAR COMPOUNDS
- URL: https://ter-arkhiv.ru/0023-4761/article/view/673174
- DOI: https://doi.org/10.31857/S0023476124030067
- EDN: https://elibrary.ru/XOPTJS
- ID: 673174
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Abstract
The crystal structure of the metallocarboxypeptidase T from Thermoactinomyces vulgaris complex with L-phenylactate was obtained with a resolution of 1.73 Å. Unlike pancreatic carboxypeptidase A, which binds one L-phenylactate molecule, in complex with CPT, the ligand occupies both S1 and S1ʹ sites of the active center simultaneously. In this case, conformational changes occur that differ from the changes caused by the alternate occupation of the S1 and S1ʹ sites by BOC-leucine and benzylsuccinic acid. These changes concern the residues E277, E59, L254, G192, S127 and Y218 and reach a span of 0.77 Å. A conclusion is made about the possible role of these residues in the recognition and catalysis of substrates by carboxypeptidase T.
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About the authors
V. Kh. Akparov
Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics of NRC “Kurchatov Institute”
Author for correspondence.
Email: valery.akparov@yandex.ru
Russian Federation, Moscow
G. E. Konstantinova
Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics of NRC “Kurchatov Institute”
Email: valery.akparov@yandex.ru
Russian Federation, Moscow
V. I. Timofeev
Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics of NRC “Kurchatov Institute”
Email: valery.akparov@yandex.ru
Russian Federation, Moscow
M. B. Shvetsov
Moscow Institute of Physics and Technology
Email: valery.akparov@yandex.ru
Russian Federation, Dolgoprudny
I. P. Kuranova
Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics of NRC “Kurchatov Institute”; Moscow Institute of Physics and Technology
Email: valery.akparov@yandex.ru
Russian Federation, Moscow; Dolgoprudny
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