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The development of antibiotic resistance of the probiotic strain Lactiplantibacillus plantarum 8P-A3 is associated with changes in the structure of extracellular vesicules and the character of their effect on bacterial biofilms
- Authors: Chernova O.A.1, Kayumov A.R.2, Markelova M.I.1,2, Salnikov V.V.1, Kutyreva M.P.2, Khannanov A.A.2, Fedorova M.S.2, Zhuravleva D.E.2, Baranova N.B.1,2, Faizullin D.A.1, Zuev Y.F.1, Chernov V.M.1
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Affiliations:
- Kazan Institute of Biochemistry and Biophysics of Kazan Science Centre of the Russian Academy of Science
- Kazan Federal University
- Issue: Vol 519, No 1 (2024)
- Pages: 35-41
- Section: Articles
- URL: https://ter-arkhiv.ru/2686-7389/article/view/651378
- DOI: https://doi.org/10.31857/S2686738924060059
- ID: 651378
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Abstract
For the first time, it was shown that the development of resistance to antibiotics (amoxicillin and clarithromycin) in vitro in the probiotic strain Lactiplantibacillus plantarum 8p-a3, associated with large-scale genomic rearrangements, a change in the profile of phenotypic sensitivity to antimicrobials of different groups, and the evolution of virulence, is also accompanied by significant changes in the lactobacillus-derived extracellular membrane vesicles transferring lipids, polysaccharides, proteins, and nucleic acids. The changes are related to the structure and cargo of vesicles, as well as their activity against biofilms of opportunistic bacteria. The data obtained are relevant for understanding the molecular mechanisms of survival of microorganisms under the selective pressure of antimicrobials, the functional potential of the probiotic vesicles and assessing their safety.
About the authors
O. A. Chernova
Kazan Institute of Biochemistry and Biophysics of Kazan Science Centre of the Russian Academy of Science
Email: kairatr@yandex.ru
Russian Federation, Kazan
A. R. Kayumov
Kazan Federal University
Author for correspondence.
Email: kairatr@yandex.ru
Russian Federation, Kazan
M. I. Markelova
Kazan Institute of Biochemistry and Biophysics of Kazan Science Centre of the Russian Academy of Science; Kazan Federal University
Email: kairatr@yandex.ru
Russian Federation, Kazan; Kazan
V. V. Salnikov
Kazan Institute of Biochemistry and Biophysics of Kazan Science Centre of the Russian Academy of Science
Email: kairatr@yandex.ru
Russian Federation, Kazan
M. P. Kutyreva
Kazan Federal University
Email: kairatr@yandex.ru
Russian Federation, Kazan
A. A. Khannanov
Kazan Federal University
Email: kairatr@yandex.ru
Russian Federation, Kazan
M. S. Fedorova
Kazan Federal University
Email: kairatr@yandex.ru
Russian Federation, Kazan
D. E. Zhuravleva
Kazan Federal University
Email: kairatr@yandex.ru
Russian Federation, Kazan
N. B. Baranova
Kazan Institute of Biochemistry and Biophysics of Kazan Science Centre of the Russian Academy of Science; Kazan Federal University
Email: kairatr@yandex.ru
Russian Federation, Kazan; Kazan
D. A. Faizullin
Kazan Institute of Biochemistry and Biophysics of Kazan Science Centre of the Russian Academy of Science
Email: kairatr@yandex.ru
Russian Federation, Kazan
Y. F. Zuev
Kazan Institute of Biochemistry and Biophysics of Kazan Science Centre of the Russian Academy of Science
Email: kairatr@yandex.ru
Russian Federation, Kazan
V. M. Chernov
Kazan Institute of Biochemistry and Biophysics of Kazan Science Centre of the Russian Academy of Science
Email: kairatr@yandex.ru
Russian Federation, Kazan
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