Исследование механизма генерации мембранного потенциала гем-медными дыхательными оксидазами в режиме реального времени

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Аннотация

Гем-медные дыхательные оксидазы являются высокоэффективными молекулярными машинами. Эти мембранные ферменты катализируют конечный этап клеточного дыхания эукариот и многих прокариот: перенос электронов от цитохромов или хинолов к молекулярному кислороду и восстановление O2 до воды. Высвобождаемая в этой окислительно-восстановительной реакции свободная энергия преобразуется гем-медными дыхательными оксидазами в трансмембранный градиент электрохимического потенциала ионов водорода (ΔµH+). Гем-медные дыхательные оксидазы обладают уникальным механизмом генерации ΔµH+ - редокс-сопряженной протонной помпой. Использование в исследовании гем-медных оксидаз комбинации прямого электрометрического метода измерения кинетики генерации мембранного потенциала с подходами и методами предстационарной кинетики и направленного мутагенеза позволяет получать уникальную информацию о перемещении протонов внутри белка в режиме реального времени. В обзоре суммированы результаты применения разрешенной во времени электрометрии к расшифровке механизмов работы этих важнейших биоэнергетических ферментов.

Об авторах

С. А Силецкий

Научно-исследовательский институт физико-химической биологии имени А.Н. Белозерского, Московский государственный университет имени М.В. Ломоносова

Email: siletsky@belozersky.msu.ru
119991 Москва, Россия

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