Аффинные якоря: сравнение популярных тегов в современной молекулярной биологии

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В функционировании живых систем ключевую роль играют межмолекулярные контакты, осуществляемые в том числе через белок-белковые взаимодействия. Одним из основных методов их изучения является аффинная очистка, сопряженная с масс-спектрометрией (AP-MS). Этот подход невозможен без совершенствования эпитопных меток, представляющих собой короткие генетические модификации белков, за которые впоследствии удается “выловить” целевые молекулы. Обзор направлен на сравнение самых популярных эпитопных меток, а также на обсуждение традиционных и инновационных исследований, в которых они применяются. Основная задача данной работы — оценить, насколько близко научное сообщество к созданию “оптимальной” метки, удовлетворяющей потребностям большинства исследователей, применяющих теги для решения широкого спектра молекулярно-биологических задач.

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П. А. Крюкова

Научно-исследовательский институт биомедицинской химии им. В.Н. Ореховича

Email: inst@ibmc.msk.ru
Россия, Москва

О. И. Киселева

Научно-исследовательский институт биомедицинской химии им. В.Н. Ореховича

Email: inst@ibmc.msk.ru
Россия, Москва

И. Ю. Курбатов

Научно-исследовательский институт биомедицинской химии им. В.Н. Ореховича

Email: inst@ibmc.msk.ru
Россия, Москва

Е. В. Поверенная

Научно-исследовательский институт биомедицинской химии им. В.Н. Ореховича

Автор, ответственный за переписку.
Email: inst@ibmc.msk.ru
Россия, Москва

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