Follow-up study of expression of Bcl-2, Bax, p53 and ACE in CD34+ cells of peripheral blood and bone marrow in acute leukemia patients in the course of induction chemotherapy
- Authors: Khodunova E.E.1, Parovichnikova E.N.1, Gal'tseva I.V.1, Kulikov S.M.1, Isaev V.G.1, Savchenko V.G.1, Khodunova EE2, Parovichnikova EN2, Galtseva IV2, Kulikov SM2, Isaev VG2, Savchenko VG2
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Affiliations:
- Hematological Research Center, Moscow
- Issue: Vol 83, No 7 (2011)
- Pages: 32-37
- Section: Editorial
- URL: https://ter-arkhiv.ru/0040-3660/article/view/30870
- ID: 30870
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Abstract
Material and methods. Flow cytoflowmetry estimated expression of Bcl-2, Bax, p53 and ACE in the cells CD34+ of BM and PB from 10 AL (4 AML and 6 ALL) patients. PB and BM samples were studied before polychemotherapy (PCT) and in the course of induction treatment: on day +8, +21 (blood only), +36 - 38. Control group consisted of 4 BM donors.
Results. The number of CD34+ cells expressing Bcl-2 in AL patients was 46,5 ± 9,35 % in BM and 39,4 + 10,8 % in PB, in healthy donors - 9 and 32,8 %, respectively. Bax expression in AL patients' cells CD34+ of BM versus this expression in donors was 3 times higher (36,7 ± 8,1 and 14,8%, respectively), of PC - 2 times lower (40,7 ± 6,59 and 75,8%, respectively). Expression of p53 in AL patients was 36,8 ± 9 % in BM and 26 ± 7,4 % in PB, in donors - 28,2 and 65 %, respectively. ACE expression on the cells CD34+ in AL patients in early disease was 62 ± 7,57 % in BM and 48 ± 8,1 % in PB, in donors - 40 and 85 %, respectively. Moreover, there were significant changes in expression of Bcl-2 in BM and Bax, ACE and p53 in PB in the cells CD34+ in AL patients during and after induction PCT.
Conclusion. The above changes evidence for unbalance of pro- and antiapoptosis proteins of regulators in AL patients. PCT changes profile of expression of these proteins, but not to the level of healthy donors.
Keywords
About the authors
Elena Evgen'evna Khodunova
Email: Hodunova_Lena@mail.ru
Elena Nikolaevna Parovichnikova
Email: elenap@blood.ru
Irina Vladimirovna Gal'tseva
Sergey Mikhaylovich Kulikov
Valentin Grigor'evich Isaev
Valeriy Grigor'evich Savchenko
E E Khodunova
Hematological Research Center, MoscowHematological Research Center, Moscow
E N Parovichnikova
Hematological Research Center, MoscowHematological Research Center, Moscow
I V Galtseva
Hematological Research Center, MoscowHematological Research Center, Moscow
S M Kulikov
Hematological Research Center, MoscowHematological Research Center, Moscow
V G Isaev
Hematological Research Center, MoscowHematological Research Center, Moscow
V G Savchenko
Hematological Research Center, MoscowHematological Research Center, Moscow
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