Adiponectin gene expression in local fat depots in patients with coronary heart disease depending on the degree of coronary lesion

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Abstract


Aim. To determine the dependence of adiponectin gene expression by subcutaneous, epicardial and perivascular adipocytes on the degree of coronary lesion in coronary heart disease.

Materials and methods. 84 patients with coronary artery disease were examined. Of these, 39 people showed a moderate degree of atherosclerotic lesion of the coronary bed (less than or equal to 22 points) on the SYNTAX Score scale, 20 – severe (22–31 points), and 25 – extremely severe (more than 32 points). Upon admission to the hospital, all patients underwent an echocardiographic study (Echocardiography, Acuson, Germany) with the calculation of the ejection fraction (EF) of the left ventricle (LV) to assess its systolic function. During a planned surgical intervention (coronary bypass surgery, CABG), adipocytes of subcutaneous, epicardial (EAT) and perivascular adipose tissue (PVAT) were taken. Adiponectin gene expression was evaluated by polymerase chain reaction (real-time PCR) using TaqMan probes. Statistical analysis was performed using Statistica 9.0.

Results. The maximum level of adiponectin expression was detected in adipocytes of PVAT, and the minimum – EAT. With an increase in the degree of atherosclerotic lesion of the coronary bed, the expression of the adiponectin gene in adipocytes of local depots significantly decreases r=-0.82; p=0.023. Moreover, the low level of gene expression in EAT correlated with a decrease in LV EF by r=0.73; p=0.03. In adipocytes of subcutaneous and especially PVAT, gene expression was the highest in patients with a moderate degree of coronary lesion.

Conclusions. Low adiponectin gene expression in EAT is associated with an increase in the degree of atherosclerotic lesion of the coronary bed and a decrease in LV EF.


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About the authors

E. V. Belik

Research Institute for Complex Issues of Cardiovascular Disease

Author for correspondence.
Email: sionina.ev@mail.ru
ORCID iD: 0000-0003-3996-3325

Russian Federation, Kemerovo

мл.н.с. лаб. исследований гомеостаза отд. диагностики сердечно-сосудистых заболеваний

O. V. Gruzdeva

Research Institute for Complex Issues of Cardiovascular Disease

Email: sionina.ev@mail.ru
ORCID iD: 0000-0002-7780-829X

Russian Federation, Kemerovo

д.м.н., зав. лаб. исследований гомеостаза отд. диагностики сердечно-сосудистых заболеваний

O. E. Akbasheva

Siberian State Medical University

Email: sionina.ev@mail.ru
ORCID iD: 0000-0003-0680-8249

Russian Federation, Tomsk

д.м.н., проф. каф. биохимии и молекулярной биологии с курсом клинической лабораторной диагностики, заместитель декана медико-биологического фак-та

Yu. A. Dyleva

Research Institute for Complex Issues of Cardiovascular Disease

Email: sionina.ev@mail.ru
ORCID iD: 0000-0002-6890-3287

Russian Federation, Kemerovo

к.м.н., с.н.с. лаб. исследований гомеостаза отд. диагностики сердечно-сосудистых заболеваний

D. A. Borodkina

Research Institute for Complex Issues of Cardiovascular Disease

Email: sionina.ev@mail.ru
ORCID iD: 0000-0002-6221-3509

Russian Federation, Kemerovo

к.м.н., с.н.с. лаб. исследований гомеостаза отд. диагностики сердечно-сосудистых заболеваний

M. Yu. Sinitsky

Research Institute for Complex Issues of Cardiovascular Disease

Email: sionina.ev@mail.ru
ORCID iD: 0000-0002-4824-2418

Russian Federation, Kemerovo

к.б.н., с.н.с. лаб. геномной медицины отд. экспериментальной и клинической кардиологии

A. V. Sotnikov

Research Institute for Complex Issues of Cardiovascular Disease

Email: sionina.ev@mail.ru
ORCID iD: 0000-0002-1828-5652

Russian Federation, Kemerovo

врач сердечно-сосудистый хирург кардиохирургического отд-ния

K. A. Kozyrin

Research Institute for Complex Issues of Cardiovascular Disease

Email: sionina.ev@mail.ru

Russian Federation, Kemerovo

врач сердечно-сосудистый хирург кардиохирургического отд-ния

N. K. Brel

Research Institute for Complex Issues of Cardiovascular Disease

Email: sionina.ev@mail.ru
ORCID iD: 0000-0002-5643-4022

Russian Federation, Kemerovo

врач-рентгенолог отд-ния лучевой диагностики

D. Yu. Naumov

Research Institute for Complex Issues of Cardiovascular Disease

Email: sionina.ev@mail.ru

Russian Federation, Kemerovo

лаборант-исследователь лаб. интервенционных методов диагностики и лечения

A. A. Shilov

Research Institute for Complex Issues of Cardiovascular Disease

Email: sionina.ev@mail.ru
ORCID iD: 0000-0002-4639-7416

Russian Federation, Kemerovo

к.м.н., с.н.с. лаб. интервенционных методов диагностики и лечения

E. E. Bychkova

Research Institute for Complex Issues of Cardiovascular Disease

Email: sionina.ev@mail.ru
ORCID iD: 0000-0002-0500-2449

Russian Federation, Kemerovo

лаборант-исследователь лаб. исследований гомеостаза отд. диагностики сердечно-сосудистых заболеваний

V. N. Karetnikova

Research Institute for Complex Issues of Cardiovascular Disease

Email: sionina.ev@mail.ru
ORCID iD: 0000-0002-9801-9839

Russian Federation, Kemerovo

д.м.н., проф., зав. лаб. патофизиологии мультифокального атеросклероза отд. мультифокального атеросклероза

O. L. Barbarash

Research Institute for Complex Issues of Cardiovascular Disease

Email: sionina.ev@mail.ru
ORCID iD: 0000-0002-4642-3610

Russian Federation, Kemerovo

чл.-кор. РАН, д.м.н., проф., дир.

References

  1. Fantuzzi G, Mazzone T. Adipose tissue and atherosclerosis: exploring the connection. Arterioscler Thromb Vasc Biol. 2007;27(5):996-1003. doi: 10.1161/atvbaha.106.131755
  2. Neeland IJ, Patel RS, Eshtehardi P, et al. Coronary angiographic scoring systems: An evaluation of their equivalence and validity. Am Heart J. 2012;164:547-52. doi: 10.1016/j.ahj.2012.07.007
  3. Sianos G, Morel MA, Kappetein AP, et al. The SYNTAX Score: an angiographic tool grading the complexity of coronary artery disease. EuroInterv. 2005;1:219-27.
  4. Груздева О.В., Акбашева О.Е., Дылева Ю.А. и др. Адипокиновый и цитокиновый профили эпикардиальной и подкожной жировой ткани у пациентов с ишемической болезнью сердца. Бюллетень экспериментальной биологии и медицины. 2017;63(5):560-3 [Gruzdeva OV, Akbasheva OE, Dyleva YuA, et al. Adipokine and cytokine profiles of epicardial and subcutaneous adipose tissue in patients with coronary artery disease. Bulletin of Experimental Biology and Medicine. 2017;63(5):560-3 (In Russ.)].
  5. Дылева Ю.А., Груздева О.В., Белик Е.В. и др. Экспрессия гена и содержание адипонектина в жировой ткани у пациентов с ишемической болезнью сердца. Биомедицинская химия. 2019;65(3):239-44 [Dyleva YuA, Gruzdeva OV, Belik EV, et al. Gene expression and adiponectin content in adipose tissue in patients with coronary artery disease. Biomeditsinskaya Khimiya. 2019;65(3):239-44 (In Russ.)].
  6. Kershaw EE, Flier JS. Adipose tissue as an endocrine оrgan. J Clin Endocrinol Metabolism. 2004;89(6):2548-56. doi: 10.1210/jc.2004-0395
  7. Pineiro R, Iglesias MJ, Gallego R, et al. Adiponectin is synthesized and secreted by human and murine cardiomyocytes. FEBS Lett. 2005;579:5163-9. doi: 10.1016/j.febslet.2005.07.098
  8. Libby P. Inflammation in atherosclerosis. Nature. 2002;420(6917):868-74. doi: 10.1038/nature01323
  9. Chandran M, Phillips SA, Ciaraldi T, Henry RR. Adiponectin: more than just another fat cell hormone? Diabetes Care. 2003;26:2442-50. doi: 10.2337/diacare.26.8.2442
  10. Kumada M, Kihara S, Ouchi N, et al. Adiponectin specifically increased tissue inhibitor of metalloproteinase-1 through interleukin-10 expression in human macrophages. Circulation. 2004;109:2046-9. doi: 10.1161/01.cir.0000127953.98131.ed
  11. Koenig W, Khuseyinova N, Baumert J, et al. Serum concentrations of adiponectin and risk of type 2 diabetes mellitus and coronary heart disease in apparently healthy middle-aged men: results from the 18-year follow-up of a large cohort from southern Germany. J Am Coll Cardiol. 2006;48:1369-77. doi: 10.1016/j.jacc.2006.06.053
  12. Pischon T. Plasma adiponectin levels and risk of myocardial infarction in men. JAMA. 2004;291:1730-7. doi: 10.1001/jama.291.14.1730
  13. Kumada M, Kihara S, Sumitsuji S, et al. Association of hypoadiponectinemia with coronary artery disease in men. Arterioscler Thromb Vasc Biol. 2003;23:85-9. doi: 10.1161/01.atv.0000048856.22331.50
  14. Matthias B, Shai I, Rimm E. Adiponectin and Future Coronary Heart Disease Events Among Men With Type 2 Diabetes. Am J Epidem. 2007;165:164-74.
  15. Wannamethee SG, Whincup PH, Lennon L, Sattar N. Circulating adiponectin levels and mortality in elderly men with and without cardiovascular disease and heart failure. Arch Intern Med. 2007;167:1510-7. doi: 10.1001/archinte.167.14.1510
  16. George J. Circulating adiponectin levels predict outcome in patients with severe congestive heart failure. Heart. 2006;92:1420-4. doi: 10.1136/ hrt.2005.083345
  17. Kistorp C, Faber J, Galatius S, et al. Plasma adiponectin, body mass index, and mortality in patients with chronic heart failure. Circulation. 2005;112:1756-62. doi: 10.1161/circulationaha.104.530972
  18. Vela D, Buja LM, Madjid M, et al. The role of periadventitial fat in atherosclerosis: An adipose subset with potential diagnostic and therapeutic implications (Review). Arch Pathol Lab Med. 2007;131(3):481-7.
  19. Sacks H, Fain J. Human epicardial adipose tissue: A review. Am Heart J. 2007;153(6):907-17. doi: 10.1016/j.ahj.2007.03.019
  20. Iacobellis G, Pistilli D, Gucciardo M, Leonetti F. Adiponectin expression in human epicardial adipose tissue in vivo is lower in patients with coronary artery disease. Cytokine. 2005;29(6):251-5. doi: 10.1016/ j.cyto.2004.11.002
  21. Bambacea C, Telescab M, Zoicoa E, et al. Adiponectin gene expression and adipocyte diameter: a comparison between epicardial and subcutaneous adipose tissue in men. Cardiovasc Pathology. 2011;20: е153–e156. doi: 10.1016/j.carpath.2010.07.005
  22. Nacci C, Leo V, De Benedictis L, et al. Infliximab therapy restores adiponectin expression in perivascular adipose tissue and improves endothelial nitric oxide-mediated vasodilation in mice with type 1 diabetes. Vasc Pharmacol. 2016;87:83-91. doi: 10.1016/j.vph.2016.08.007
  23. Yamauchi T, Kamon J, Waki H, et al. Globular adiponectin protected ob/ob mice from diabetes and ApoE-deficient mice from atherosclerosis. J Biol Chem. 2003;278:2461-8. doi: 10.1074/jb
  24. Eiras S, Teijeira-Fernández E, Shamagian LG, et al. Extension of coronary artery disease is associated with increased IL-6 and decreased adiponectin gene expression in epicardial adipose tissue. Cytokine. 2008;43(2):174-80. doi: 10.1016/j.cyto.2008.05.006
  25. Chatterjee TK, Stoll LL, Denning GM, et al. Proinflammatory phenotype of perivascular adipocytes: influence of high-fat feeding. Circ Res. 2009;104:541-9. doi: 10.1161/circresaha.108.182998
  26. Maahs DM, Ogden LG, Kinney GL, et al. Low plasma adiponectin levels predict progression of coronary artery calcification. Circulation. 2005;111:747-53. doi: 10.1161/01.cir.0000155251.03724.a5
  27. Araki T, Emoto M, Teramura M, et al. Effect of adiponectin on carotid arterial stiffness in type 2 diabetic patients treated with pioglitazone and metformin. Metabolism. 2006;55:996-1001. doi: 10.1016/j.metabol. 2006.03.008

Supplementary files

Supplementary Files Action
1.
Fig. 1. Expression of the adiponectin gene in the culture medium of subcutaneous, epicardial and perivascular adipocytes in patients with coronary artery disease.

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2.
Fig. 1. Expression of the adiponectin gene in the culture medium of subcutaneous, epicardial and perivascular adipocytes during primary isolation, depending on the degree of damage to the coronary bed.

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