Prevalence ofmycelial fungi in a hematological hospital

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Aim. To study prevalence, quantity and species of mycelial fungi in the air of a hematological hospital.
Material and methods. Air samples in the hematological departments were taken monthly by PU- IB
device in the volume 250 l/min. Seeding and identification of mycelial fungi were made on the
Chapeck medium.
Results. Tests for mycelial fungi in the air was 95%. Dominating species were the following: Penicillium
spp., Cladosporium spp., Aspergillus spp. The fungal contamination was seasonal. Maximal isolation
of Penicillium spp was seen in winter and autumn. Aspergillus spp. is represented with 13 species
of which most frequent were A.versicolor, A. niger, A. fumigatus, A. ochraceus, A. flavus. Pathological
material from 19 patients contained Aspergillus spp.: A. fumigatus, A. flavus, A. niger. The isolation
peak was in autumn-winter and coincided with maximal isolation of fungal spores from the air. The
analysis of air samples taken in the wards where patients had Aspergillus spp. In biomaterials showed
that concentration of spores Aspergillus spp. was higher than in nearby wards and corridor: 45.6 CPU/M3 vs 18.8
CFU/m3 and 24.7 CFU/M3, respectively. However, morphologically identical strains (patient-air)
were recognized only in 4 cases from the nearby wards and corridor. None of the air samples
taken in the ward of the patient contained identical species. Minimal amount of the spores of micromycetes
was registered in the wards furnished with ventilation with laminar airflow. Most contaminated
was the air of 4 bed wards in the old building.
Conclusion. Mycological monitoring of the air in hematological departments determined the structure
of mycelial fungi complexes. Though ambient air in the wards is full of fungi, detection of morphologically
identical strains causing invasive mycosis in immunocompromised patients is rare. Further studies
are necessary.


  1. Singh N. Trends in the epidemiology of opportunistic fungal infections: Predisposing factors and the impact of antimicrobial use practices. Clin. Infect. Dis. 2002; 33: 1692-1696.
  2. Morris G., Kokki M. H., Richardson M. D. Sampling of aspergillus spores in the air. Glasgow. 2000.
  3. Munos P., Burillo A., Bouza E. Environmental surveillance and other control measures in the prevention of nosocomial fungal infections. Clin. Microbiol. Infect. Dis. 2001; 7: 38-45.
  4. Anaissie E. J., Stratton S. L., Dignani С. et al. Pathogenic aspergillus species recovered from A hospital water system: A 3- year prospective study. Clin. Infect. Dis. 2002; 34: 780-789.
  5. Anaissie E. J., Kuchar R. Т., Rex J. H. et al. Fusariosis associated with pathogenic fusarium species colonization of a hospital water system: A new paradigm for the epidemiology of opportunistic mold infections. Ibid. 33: 1871 - 1878.
  6. Revancar S. G., Patterson J., Sutton D. et al. Disseminated phaephymycosis: Review of an emerging mycosis. Ibid. 34: 467-476.
  7. Nucci M., Akiti Т., Barreiros G. et al. Nosocomial outbrek of exophiala jenselmei fungemia associated with contamination of hospital water. Ibid. 1475-1480.
  8. Hoog G. S., Guarro J., Gene J., Figueras M. J. Atlas of clinical fungi. 2-nd ed. Spain. 2000. 39.
  9. Fridkin S. K., Jarvis W. R. Epidemiology of nosocomial fungal infections. Clin. Microbiol. Rev. 1996; 9(6): 499-511.
  10. Bouakline A., Lacroix C., Roux N. et al. Fungal contamination of food distributed to neutropenic patients. In: 39-th Interscience conference on antimicrobial agents and chemotherapy. Sanfrancisco 1999. 560.
  11. Jarv H., Naaber P., Koljalg S. et al. Surveillance of Aspergillus spp. air contamination in a hematology and transplant department. Clin. Microbiol. Infect. 2001; 7 (suppl. 1): 394, abstr. 696.
  12. Panagopoulou P., Filioti I., Farmaki E. et al. Environmental surveillance for filamentous in a tertiary care hospital. Ibid. 394.
  13. Марфенина О. Е., Кулько Ф. Б., Иванова А. Е., Согонов М. В. Микроскопические грибы во внешней среде города. Микол. и фитопатол. 2002; 4: 22-32.
  14. Жданова Я. Н., Васильева А. И. Меланинсодержащие грибы в экстремальных условиях. Киев: Наукова думка; 1988.
  15. Cimon В., Zouhair R., Sytnoens F. et al. Aspergillus terreus in a cystic fibrosis clinic: environmental distribution and patient colonization pattern. J. Hosp. Infect. 2002; 10: 81-82.
  16. Perfect J. R., Cox G. M., Lee С. A. et al. The impact of isolation of aspergillus species: A hospital-based survey of aspergillosis. Clin. Infect. Dis. 2002; 33: 1824-1833.
  17. Ильина В. Я., Богомолова Т. С., Чилина Г. А. Микроэкология больничных помещений. В кн.: I Съезд микологов России: Тезисы докладов. М. 2002. 54.
  18. Latge J. P. Aspergillus fumigatus and aspergillosis. Clin. Microbiol. Rev. 1999; 12: 310-350.
  19. Anaissie E. J., Stratton S. L., Summerbell R. C. et al. Aspergillus species in hospitals: Showering as a potential mode of exposure. In: 39-th Interscience conference on antimicrobial agents and chemotherapy. Sanfrancisco. 1999. 578, abstr. 1908.

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