The specific features of the immunophenotype of blast cells in patients with de novo normal karyotype acute myeloid leukemia and FLT3-ITD mutation

Abstract

AIM: To study the specific features of de novo acute myeloid leukemia (AML) with FLT3-ITD mutation/MATERIAL AND METHODS: The results of examination were analyzed in 101 patients. Bone marrow morphological specimens were stained with Pappenheim stain. The karyotype was investigated using the standard GTG-banding method. Blast cells were immunotyped in a five-color analysis on a Cytomics FC 500 laser flow cytofluorometer/RESULTS: FLT3-ITD mutation was identified in 21 patients who had a varying morphological nature of blasts, different karyotype variants, and frequently additional NPM1 gene mutation. The distinctive property of 10 patients with normal karyotype and FLT3-ITD mutation (without NPM1 gene mutation) was the larger number of cases with high expression of HLA-DR and CD7 than in the control group that included 18 patients with normal karyotype AML without FLT3-ITD nutation: 50% versus 6.2% (p=0.007) and 100% versus 55.6% (p=0.014), respectively/CONCLUSION: Normal karyotype AML with FLT3-ITD mutation is a group that is homogeneous in the biological phenotype of leukemia cells.

Full Text

Особенности иммунофенотипа бластных клеток у больных de novo острым миелоидным лейкозом с нормальным кариотипом и мутацией FLT3-ITD. - Резюме. Цель исследования. Изучение особенностей de novo острого миелоидного лейкоза (ОМЛ) с мутацией FLT3-ITD. Материалы и методы. Проведен анализ результатов обследования 101 больного. Морфологические препараты костного мозга окрашивали по Паппенгейму. Кариотип изучали с помощью стандартного GTG-метода. Иммунофенотипирование бластных клеток проводили в пятицветном анализе на лазерном проточном цитофлуориметре Cytomics FC 500. Результаты. Мутация FLT3-ITD обнаружена у 21 больного, которые имели разную морфологическую природу бластов, разные варианты кариотипа и нередко дополнительную мутацию гена NPM1. Отличительной особенностью у 10 больных с нормальным кариотипом и мутацией FLT3-ITD (без мутации гена NPM1) было увеличение числа случаев с высокой экспрессией HLA-DR и CD7 по сравнению с контрольной группой, в которую включены 18 больных ОМЛ с нормальным кариотипом и без мутации FLT3-ITD: 50% против 6,2% (р=0,007) и 100% против 55,6% (р=0,014) соответственно. Заключение. ОМЛ с нормальным кариотипом и мутацией FLT3-ITD представляет группу, однородную по биологическому фенотипу лейкозных клеток.
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Copyright (c) 2014 Gritsaev S.V., Martynkevich I.S., Chubukina Z.V., Petrova E.V., Kostroma I.I., Ivanova M.P., Martynenko L.S., Potikhonova N.A., Bubnova L.N., Abdulkadyrov K.M.

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