Neutrophil bactericidal and absorptive function in gout
- Authors: Khitrik N.M.1, Didkovskiy N.A.1, Malashenkova I.K.1, Tsurko V.V.1, Egorov I.V.1, Khitrik NM1, Didkovsky NA1, Malashenkova IK1, Tsurko VV1, Egorov IV1
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Affiliations:
- Issue: Vol 81, No 10 (2009)
- Pages: 49-52
- Section: Editorial
- URL: https://ter-arkhiv.ru/0040-3660/article/view/30525
- ID: 30525
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Abstract
Subjects and methods. The study included 48 male patients with a valid diagnosis of gout (mean age, 59.7 ± 10.3 years; duration of the disease, 9.2 ± 2.1 years; blood uric acid (UA), 520 ± 80 μmol) (Group 1); 25 apparently healthy volunteers (mean age 55.1 ± 6.2 years; blood UA, 350 μmol/l) (Group 2). Neutrophil functional activity was estimated from the phagocytic-nitroblue tetrazolium reduction (NBT test) and myeloperoxidase (MPO) activity, the determination of non-enzyme cationic proteins (CP) and their spontaneous and induced indices. Neutrophil phagocytic function was also studied.
Results. In Group 1, the content of active oxygen forms (AOF) was increased, on average, to 113.3 ± 8.65 conventional units (CI) versus 88.22 ± 5.23 CI in Group 2; p < 0.05). In 34 (70.8%) of the 48 male patients with gout, spontaneous MPO activity was significantly reduced as compared with that in Group 2: 348.4 ± 13.6 and 509.2 ± 12.2 CI, respectively (p < 0.01). In Group 1, the level of CP was noticeably decreased to 60.1 ± 2.06 CI whereas in Group 2, it was 84.91 ± 5.36 CI (p < 0.05). In gouty patients, the CP stimulation index averaged 0.70 ± 0.13; in Group 2, it was significantly higher - 1.2 ± 0.05 (p < 0.05).
Conclusion. In the majority of gouty patients, neutrophil functional activity suffers due to its lower phagocytic function, which suppresses the body's antioxidant defense and contributes to the chronic pattern of an inflammatory process.
Keywords
About the authors
Nadezhda Mikhaylovna Khitrik
Email: clinimmunolog@rambler.ru
Nikolay Antonovich Didkovskiy
Email: clinimmunolog@rambler.ru
Irina Konstantinovna Malashenkova
Email: clinimmunolog@rambler.ru
Vladimir Viktorovich Tsurko
Email: mtpndm@dol.ru
Il'ya Vladimirovich Egorov
Email: mtpndm@dol.ru
N M Khitrik
N A Didkovsky
I K Malashenkova
V V Tsurko
I V Egorov
References
- Bird L. Gouty inflammation cristal clear. Nature. Rev. Immunol. 2006; 9 (6): 627-628.
- Choi H. K., Curhan G. Gout: epidemiology and lifestyle choices. Curr. Opin. Rheumatol. 2005; 17: 341-345.
- Zhang W., Doherty M., Bardin T. et al. EULAR evidence based recommendations for gout. Ann. Rheum. Dis. 2006; 65: 1312-1324.
- Landis R. C., Yagnik D. R. et al. Safe disposal of inflammatory monosodium urate monohydrate crystals by differentiated macrophages. Arthr. and Rheum. 2002; 48 (11): 3026-3033.
- Wallace S. L., Robinson H., Masi A. T. et al. Preliminary criteria for the classification of the acute arthritis of primary gout. Arthr. and Rheum. 1977; 20 (9): 895-900.
- Arnhold J. Free radicals - friends or foes? Biochemistry 2004; 69 (1): 12-14.
- Долгушин И. И., Бухарин О. В. Нейтрофилы и гомеостаз. Екатеринбург; 2001.
- Маянский А. Н., Пикуза О. И. Клинические аспекты фагоцитоза, Казань; 1999.