A morphometric analysis of the nuclei and nucleoli in tumor cells in lymphogranulomatosis, diffuse large B-cell lymphoma and anaplastic large cell lymphoma

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Aim. To make a comparative morphometric analysis of the nuclei and nucleoli of tumor cells in lymphogranulomatosis (LGM), diffuse large B-cell lymphoma (DLBCL) and anaplastic large cell lymphoma (ALCL) for differential diagnosis of these lymphomas.
Material and methods. Biopsy material (lymph node biopsies) was frozen in hexan, fixed and stained, then microscopic pictures were made.
Results. Mean area of tumor cell nuclei in LGM was 97.25 ± 68.77 mcm2, in DLBCL and ALCL - 55.89 ± 20.13 mcm2 and 70.31 ± 34.64 mcm2, respectively. The area differences were significant (p < 0.001). Hodgkins and Berezovsky-Rid-Sternberg cell bucleoli area was the largest (11.44 ± 7.83 mcm2). The nucleoli of the former are larger than those of the latter. Mean area of the nucleoli in DLBCL was 3.05 ± 1.58, in ALCL - 5.53 ± 4.94 mcm2. The differences are significant (p < 0.001).
Conclusion. Nucleoli in Hodgkins cells are significantly larger than those in the tumor cells in ALCL and DLBCL and the nucleoli with the area more than 12 mcm2 can be used in differential diagnosis between LGM and DLBCL but not between LGM and ALCL.


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