Functional role of Bap170 domains in enhancer-dependent gene activity in Drosophila Melanogaster

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The Bap170 subunit of the SWI/SNF chromatin remodeler exhibits activator functions when artificially recruited to the LacZ reporter promoter in enhancer-dependent transcription. In this study, the functional significance of Bap170 protein domains in reporter activation was analyzed. Deletion of the ARID domain does not reduce Bap170 activity. Increased expression of the LacZ reporter was observed in the form of Bap170 without a region that includes LXXLL motifs. Deletions of the central (RFX domain and IDRs) and C-terminal region (zinc fingers) lead to a significant decrease in transgene expression. Apparently, these regions of Bap170 are critical for the function of this protein in activated transcription.

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作者简介

V. Chmykhalo

Institute of Gene Biology, Russian Academy of Sciences

编辑信件的主要联系方式.
Email: vkchmykhalo@icloud.com

Laboratory of Gene Expression Regulation in Development

俄罗斯联邦, Moscow

D. Amendola

Università di Napoli Federico II

Email: vkchmykhalo@icloud.com
意大利, Naples

Y. Shidlovskii

Institute of Gene Biology, Russian Academy of Sciences

Email: vkchmykhalo@icloud.com

Laboratory of Gene Expression Regulation in Development

俄罗斯联邦, Moscow

L. Lebedeva

Institute of Gene Biology, Russian Academy of Sciences

Email: vkchmykhalo@icloud.com

Laboratory of Gene Expression Regulation in Development

俄罗斯联邦, Moscow

P. Schedl

Princeton University

Email: vkchmykhalo@icloud.com

foreign member of RAS

美国, Princeton

E Giordano

Università di Napoli Federico II

Email: vkchmykhalo@icloud.com
意大利, Naples

参考

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2. Fig. 1. Structure of the Bap170 protein and its mutant forms. a – Domain structure of Bap170. The regions deleted in the analyzed mutants are indicated. The numbers of amino acid residues at the boundaries of the regions are given. b – Scheme of the used model of the enhancer-dependent transgene LexAop::LacZ, expressed under the control of the fusion protein LexA-Bap170. c – The genetic constructs used and the mutant forms of Bap170 encoded by them.

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3. Fig. 2. Activity of Bap170 deletion variants in enhancer-dependent expression of the LacZ transgene in the antennal and wing imaginal discs of 3rd instar larvae. The exposure during staining is the same for all lines. wt – in this case LexA-Bap170. The enhancer localized near the insertion of the lacZ transgene is indicated on the left.

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