Мақаланы E-mail арқылы жіберу Development and evaluation of the effectiveness of a multivalent DNA vaccine against brucellosis
- Авторлар: Khodarovich Y.M.1, Elshazly M.A.2, Marzanova S.N.3, Magomedova S.3, Shell W.S.2, Nassif S.A.2, Devrishov D.A.3, Deyev S.M.1
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Мекемелер:
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
- Central Laboratory for Evaluation of Veterinary Biologics (CLEVB)-Agriculture Research Center (ARC)
- Federal State Educational Institution of Higher Professional Education Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I. Skryabin
- Шығарылым: Том 520, № 1 (2025)
- Беттер: 31-37
- Бөлім: Articles
- URL: https://ter-arkhiv.ru/2686-7389/article/view/682025
- DOI: https://doi.org/10.31857/S2686738925010062
- EDN: https://elibrary.ru/tcxfpl
- ID: 682025
Дәйексөз келтіру
Ашық рұқсат
Рұқсат берілді
Аннотация
Vaccination against brucellosis is currently carried out using attenuated strains of live brucellae, which causes serious side effects in animals and is of little use for human vaccination. In order to develop a safer vaccine against brucellosis, we created plasmid DNA capable of expressing six brucellae proteins in mammalian cells. Linear polyethyleneimine was used to increase the efficiency of DNA delivery to cells. Data on optimization of the conditions for the formation of DNA-polyethyleneimine complexes are presented. It is shown that a single intramuscular or subcutaneous injection of plasmid DNA complexes with polyethyleneimine at a low dose (20 μg/mouse) has a reliable protective effect against subsequent infection of mice with a test strain of brucellae.
Негізгі сөздер
Толық мәтін
Авторлар туралы
Y. Khodarovich
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Хат алмасуға жауапты Автор.
Email: khodarovich@mail.ru
Ресей, Moscow
M. Elshazly
Central Laboratory for Evaluation of Veterinary Biologics (CLEVB)-Agriculture Research Center (ARC)
Email: khodarovich@mail.ru
Египет, Cairo
S. Marzanova
Federal State Educational Institution of Higher Professional Education Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I. Skryabin
Email: khodarovich@mail.ru
Ресей, Moscow
S. Magomedova
Federal State Educational Institution of Higher Professional Education Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I. Skryabin
Email: khodarovich@mail.ru
Ресей, Moscow
W. Shell
Central Laboratory for Evaluation of Veterinary Biologics (CLEVB)-Agriculture Research Center (ARC)
Email: khodarovich@mail.ru
Египет, Cairo
S. Nassif
Central Laboratory for Evaluation of Veterinary Biologics (CLEVB)-Agriculture Research Center (ARC)
Email: khodarovich@mail.ru
Египет, Cairo
D. Devrishov
Federal State Educational Institution of Higher Professional Education Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I. Skryabin
Email: khodarovich@mail.ru
Corresponding Member of the RAS
Ресей, MoscowS. Deyev
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: khodarovich@mail.ru
Academician of the RAS
Ресей, MoscowӘдебиет тізімі
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