Isolation and Physicochemical Characteristics of Water-Soluble Polysaccharide from Locally Growing and Cultivated Basidium Raw Materials of Ganoderma lucidum

S. B. Khaytmetova; Хайтметова С. Б.; A. S. Turaev; Тураев А. С.; G. A. Khalilova; Халилова Г. А.; B. I. Muhitdinov; Мухитдинов Б. И.; S. R. Makkamboeva; Маккамбоева С. Р.

doi:10.31857/S2308112023600084

Isolation and Physicochemical Characteristics of Water-Soluble Polysaccharide from Locally Growing and Cultivated Basidium Raw Materials of Ganoderma lucidum

Авторлар: Khaytmetova S.B.¹, Turaev A.S.¹, Khalilova G.A.¹, Muhitdinov B.I.¹, Makkamboeva S.R.¹
Мекемелер:
1. Sadykov Institute of Bioorganic Chemistry, Academy of Sciences of Republic of Uzbekistan
Шығарылым: Том 65, № 5 (2023)
Беттер: 362-373
Бөлім: ПРИРОДНЫЕ ПОЛИМЕРЫ
URL: https://ter-arkhiv.ru/2308-1120/article/view/650812
DOI: https://doi.org/10.31857/S2308112023600084
EDN: https://elibrary.ru/DDDUQO
ID: 650812

Дәйексөз келтіру

Толық мәтін

Ашық рұқсат
Рұқсат жабық

Рұқсат берілді
Рұқсат жабық

Тек жазылушылар үшін

Аннотация
Авторлар туралы
Әдебиет тізімі
Қосымша файлдар
Статистика

Аннотация

Branched polysaccharides have been isolated from basidiomycete raw materials of locally growing and cultivated Ganoderma lucidum. It has been found that the isolated fractions contain branched polysaccharides in the form of complexes with melanin. After purification of polysaccharides by ion exchange chromatography from locally growing and cultivated basidial raw materials, two fractions have been obtained: neutral polysaccharides of locally growing Ganoderma lucidum (GW-1), cultivated Ganoderma lucidum (GWL-1) with a yield of 25.71 and 29.85%, respectively, and anionic polysaccharides of locally growing Ganoderma lucidum (GW-2), cultivated Ganoderma lucidum (GWL-2), with a yield of 5.26 and 4.19%. The physicochemical properties of the obtained samples have been studied by IR and UV spectroscopies. The purity degree of fractions of branched polysaccharides has been determined. Using gas chromatography, one-dimensional (13C NMR, 1H NMR), and two-dimensional (COSY, TOCSY, HSQC, HMBC, NOESY) NMR spectroscopies, the compositions and molecular structures of the obtained polysaccharide samples have been determined. The results showed that the isolated and purified polysaccharides are β-glucan-type branched polysaccharides that have branch point (1,4,6)- and (1,3,6)-linked glucopyranose residues.

Әдебиет тізімі

Wasser S.P., Weis A.L. // Int. J. Med. Mushr. 1999. V. 1. P. 31.
Wasser S.P. // Appl. Microbiol. Biotechnol. 2002. V. 60. P. 258.
Mizuno T. // Foods Food Ingred. J. Jpn. 1996. V. 167. P. 69.
Mizuno T. // Food Rev. Intern. 1995. V. 11. P. 173.
Gorin PAJ, Barreto-Berger E. // The Polysaccharides / Ed. by G.O. Aspinall. New York: Acad. Press, 1983. P. 365.
Liu W., Lu W., Chai L., Liu Y., Yao W., Gao H. // Carbohydr. Polym. 2017. V. 176. P. 140.
Qingbin G., Lianzhong A., Steve W., Cui Q. // Springer Briefs in Molecular Science Biobased Polymers / Ed. by P. Navard. Switzerland, 2018. P. 20.
Pan D., Wang L., Chen C., Teng B., Wang Ch., Xu Z., Hu B., Zhou P. // Food Chem. 2012. V. 135. № 3. P. 1097.
Magdeldin S. Affinity Chromatography: Principles and Applications. China: InTech, 2012.
Wang. Q., Zhao X., Pu J., Luan X. // Carbohydr. Polym. 2016. V. 143. P. 296.
Kim Y., Kim B., Cheong Ch., David L., Williams, Kim Ch., Lim S. // Carbohydr. Res. 2000. V. 328. P. 331.
Patel B.K., Campanella O.H., Janaswamy S. // Carbohydr. Polym. 2013. V. 92. P. 1873.
Tao Y., Zhang R., Wei Y., Liu H., Yang H., Zhao Q. // Carbohydr. Polym. 2015. V. 128. P. 179.
Khaytmetova S.B., Turaeva A.S., Khalilova G.A. // Polymer Science B. 2022. V. 64. № 4. P. 500.
Bjomdal H., Hellerqvist C., Lindberg B., Svensson S. // Angew. Chem. Int. Ed. Eng. 1970. V. 9. P. 610.
Arun G., Eyini M, Gunasekaran P. // J. Experim. Biol. 2015. V. 53. P. 380.
Tian Y., Elbing K., Stefan H. // Microbiology. 2008. V. 154. P. 2814.
Jianguo W., Yahong Y., Tianli Y. // Carbohydr. Polym. 2014. V. 10. P. 247.
Khalilova G.A., Turaev A.S., Muhitdinov B.I., Filatova A.V., Haytmetova S.B., Normakhamatov N.S. // Am. J. Appl. Sci. 2021. V. 3. № 1. P. 9.
Ding H.H., Cui S.W., Goff H.D., Chen J., Guo Q., Wang Q. // Carbohydr. Polym. 2016. V. 151. P. 538.
Ren Y., Bai Y., Zhang Z., Cai W., Del R., Flores A. // Molecules. 2019. V. 24. № 17. P. 3122.
Халилова Г.А., Тураев А.С., Мухитдинов Б.И., Хайтметова С.Б., Азимова Л.Б., Нормахаматов Н.С. // Докл. АН Республики Узбекистан. 2020. № 5. С. 55.