PrikladnaĆ¢ biohimiĆ¢ i mikrobiologiĆ¢, Vol 59, No 2 (2023)

Formation of Various Antimicrobial Peptide Emericellipsin Isoforms in Emericellopsis alkalina under Different Cultivation Conditions

Kuvarina A.E., Sukonnikov M.A., Rogozhin E.A., Serebryakova M.V., Timofeeva A.V., Georgieva M.L., Sadykova V.S.

Abstract


A microbiological screening of the target component of emericellipsin A of the Emericellopsis alkalina E101 strain was carried out in various biotechnological systems at various pH. The content of emericellipsin A was quantified under these conditions.It has been established that the new approved membrane-liquid cultivation method at pH 10 contributes to an increase in the yield of the main component of emericellipsin A. It was shown that the new method of cultivating the strain E. alkalina E101 also promotes the synthesis of various isoforms of the main component of emericellipsin A. Some comparative analysis of them was carried out.