Erythrocyte surface architectonics and cytoskeleton and their modulation by adrenergic agents in bronchial asthma


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Aim. To evaluate erythrocyte surface architectonics and cytoskeleton in bronchial asthma (BA) under modulation of adrenergic agentsMaterial and methods. 24 healthy persons, 61 patients with bronchial asthma (BA) were examined. Of them, 28 patients had allergic BA (ABA) and 33 ones had nonallergic BA (NABA). Erythrocyte surface architectonics was studied by phase-contrast microscopy. Fixation in 0.5% solution of glutar aldehyde was used, preparation "squashed drop" was prepared. In all preparations adrenalin hydrochloride and obzidan were used in equimolar final concentrations of 4 10~5M. The mean morphologic index of transformation that reflects the shift to stomatocytosis or to echinocytosis was estimated to characterize integrally erythrocyte surface architectonics. The cytoskeleton was studied by the modified method of Chentsov's et al. The integral optic density was evaluated on imaging analyzer of Ista-Videotest company (St-Petersburg).
Results. In healthy persons there was a correlation between erythrocyte surface architectonics and cytoskeleton under modulation of adrenergic system. Adrenalin-induced decrease of cytoskeletal proteins correlated with the shift to stomatocytosis. In NABA erythrocyte surface architectonics was characterized by a pronounced shift to stomatocytosis. This shift was accompanied with the most pronounced decrease of cytoskeletal proteins and it reduced in remission. In ABA manifestation of the shift was minimal and did not depend on the phase of the disease. In ABA no changes in erythrocyte surface architectonics in the presence of adrenalin were revealed. In the same conditions a decrease in cytoskeletal proteins was found.
Conclusion. A correlation was found between erythrocyte surface architectonics and cytoskeleton in healthy persons. In ABA, under modulation of adrenergic system by adrenalin dissociation was revealed between receptor and cytoskeletal mechanisms of cell form induction. This dissociation does not depend on the phase of the disease and is thought to be one of the important postreceptor disorders typical for this variant of the disease.

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V Mineev

Т Lalaeva

参考

  1. Минеев В. Н. Особенности мембрано-рецепторных нарушений в патогенезе аллергических заболеваний. В кн.: Федосеев Г. Б. (ред.) Общая аллергология. СПб: НормедИздат;2001;Т. 1: 504-517.
  2. Минеев В. Н. Роль эритроцитов в патогенезе бронхиальной астмы - нетрадиционные аспекты. Тер. арх. 1990; 62 (12): 119-121.
  3. Минеев В. Н., Лалаева Т. М., Скопичев В. Г. Особенности цитоскелета эритроцитов при бронхиальной астме. Пульмонология 1999; 1: 28-31.
  4. Bessis M. Red cell shapes. An illustrated classification and its rationale. Nouv. Rev. Franc. Hematol. - 1972; 12: 721-746.
  5. Ferrell J. E., Huestis W. H. Phosphoinositide metabolism and the morphology of human erythrocytes. J. Cell Biol. - 1984. - 98: 1992-1998.
  6. Reinhart W. H., Chien S. Red cell rheology in stomatocyteechinocyte transformation: roles of cell geometry and cell shape. Blood 1986; 67: 1110-1118.
  7. Чепцов Ю. С., Воробьев И. А., Надежншш Е. С. Простой способ выявления центриолей и цитоскелета в клетках культуры ткани с помощью светового микроскопа. Цитология 1982; 24 (3): 243-247.
  8. Минеев В. Н., Яблонская В. Н., Булатова Н. Ю. и др. Атипическая бронхиальная астма как патология трансмембранного проведения регуляторного сигнала. В кн.: Актуальные проблемы пульмонологии. М.; 2000. 196-204.
  9. Яковлева Н. В. Вирусиндуцированная аллергия. В кн.: Федосеев Г. Б. (ред.) Общая аллергология. СПб: НордмедИздат; 2001; Т. 1: 78-98.
  10. Козлов М. М., Лерхе Д. Г., Маркин В. С. Свободная энергия спонтанной кривизны заряженной мембраны с гликокаликсом. Биол. мембраны 1985; 2 (8): 806-812.
  11. Шубладзе А. К., Бычкова Е. Н., Баринский И. Ф. Вирусемия при острых и хронических инфекциях. М.: Медицина; 1974.
  12. Nishimura Н., Sugawara К., Kitame F., Nakamura К. Attachment of influenze C virus to human erythrocytes. J. Gen. Virol. 1988; 69 (10). 2545-2553.
  13. Georgian G. N., Morrison J. E. G., Cherry P. J. Digital fluorescence imaging of fusion of influenza virus with erythrocytes. FEBS Lett. 1989; 250 (2): 487-492.
  14. Юрлов В. М., Евдокимова Т. Б. Роль эритроцитов в патогенезе нарушений гемостаза у больных бронхиальной астмой. Клин. мед. 1986; 9: 74-77.
  15. Баранова И. А., Орлов С. Н., Петруияка В. В. и др. АТФазная активность эритроцитов больных бронхиальной астмой: влияние кальцитрина. Пульмонология 1993; (1): 53- 56.
  16. Sundgust J., Bias D., Hogan J. E. et al. The a-adrenergic receptor in human erythrocyte membranes mediates interaction in vitro of epinephrine and thyroid hormone at the membrane Ca2+-ATPase. Cell. Signal. 1992; 4 (6): 795-799.
  17. Wong P. A. Basis of echinocytosis and stomatocytosis in the disc-sphere transformations of the erythrocyte. J. Theor. Biol. 1999; 196 (1): 343-361.
  18. Орлов С. Н., Баранова И. А., Покудин Н. И. и др. Транспорт одновалентных ионов и кальция в эритроцитах больных бронхиальной астмой. Вестн. АМН СССР 1991; 3: 43-49.
  19. Szasz I., Sarcadi В., Gardos G. Mechanism of Ca2+-dependent selective rapid K+-transport induced by propranolol in red cells. J. Membrane Biol. 1977; 35: 75-93.
  20. Strader С. D., Candelore M. R., Hill W. S. A single amino acid substitution in the beta-adrenergic receptor promotes partial agonist activity from antagonists. J. Biol. Chem. 1989; 264 (28): 16470-16477.
  21. Воробей С. В., Черницкий Е. А. Структура и функция эритроцитарных мембран. Минск: Наука и техника; 1991. 92- 120.
  22. Перцева М. Н. Молекулярные основы развития гормонокомпетентности. Л.: Наука; 1989.

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