Email this article Characterization of cellular immunity parameters in children with acute viral hepatitis A
- Authors: Budarina NA1, Belaya ОF1, Chulanov VP1, Paimanov NV1, Pak SG1
-
Affiliations:
- Issue: Vol 78, No 11 (2003)
- Pages: 31-35
- Section: Editorial
- URL: https://ter-arkhiv.ru/0040-3660/article/view/29694
- ID: 29694
Cite item
Full Text
Abstract
Aim. To study the populational composition of lymphocytes and the specific features of production of
cytokines in children with acute viral hepatitis A (A VHA).
Materials and methods. 128patients aged 11 to 14 years who had AVHA, moderate AVHA being in
83.5% were examined. In 87.2% of the children, the disease was cyclic. The etiology of the disease
was verified by symultaneously detecting anti-HAV IgM (enzyme immunoassay) and by the presence
of HA V RNA (polymerase chain reaction) in the blood. Peripheral lymphocytes (CD) were phenotypes
in the indirect immunofluorescence test using monoclonal antibodies; cytokines were determined by the
enzyme immunoassay; serum p2-microglobulin was done by radioimmunoassay.
Results. The children with AVHA were found to have elevated levels of tumor necrosis p-factor, interleukin-ip (IL-ip), and IL-4 in the icteric period, as well as a decrease in their levels at convalescence. IL-6 was detected in individual patients only in the first 3 days of the icteric period. The peak
of A VHA was characterized by relative lymphocytosis, by decreases in the counts of T helper/inductor
cells and natural killer cells, by increases in the count of CD25 cells, convalescence, by preserved
lymphocytosis, by the increased levels of T lymphocytes that carry the markers CD3, CD4, CD8,
CD16, CD25, and CD95, and by higher toxicity of p2-microglobulin.
cytokines in children with acute viral hepatitis A (A VHA).
Materials and methods. 128patients aged 11 to 14 years who had AVHA, moderate AVHA being in
83.5% were examined. In 87.2% of the children, the disease was cyclic. The etiology of the disease
was verified by symultaneously detecting anti-HAV IgM (enzyme immunoassay) and by the presence
of HA V RNA (polymerase chain reaction) in the blood. Peripheral lymphocytes (CD) were phenotypes
in the indirect immunofluorescence test using monoclonal antibodies; cytokines were determined by the
enzyme immunoassay; serum p2-microglobulin was done by radioimmunoassay.
Results. The children with AVHA were found to have elevated levels of tumor necrosis p-factor, interleukin-ip (IL-ip), and IL-4 in the icteric period, as well as a decrease in their levels at convalescence. IL-6 was detected in individual patients only in the first 3 days of the icteric period. The peak
of A VHA was characterized by relative lymphocytosis, by decreases in the counts of T helper/inductor
cells and natural killer cells, by increases in the count of CD25 cells, convalescence, by preserved
lymphocytosis, by the increased levels of T lymphocytes that carry the markers CD3, CD4, CD8,
CD16, CD25, and CD95, and by higher toxicity of p2-microglobulin.
Keywords
References
- Онищенко Г. Г. Эпидемиологическая обстановка в РФ и основные направления деятельности по ее стабилизации. В кн.: Материалы к докладу на VIII Всероссийском съезде эпидемиологов, микробиологов и паразитологов. М.; 2002; 1-39.
- Соринсон С. Н. Вирусные гепатиты. Л.: Медицина; 1987.
- Кетлинский С. А., Симбирцев А. С, Воробьев А. А. Эндогенные иммуномудуляторы. СПб: Гиппократ; 1992.
- Фрейдлин И. С. Ключевая позиция макрофагов в цитокиновой регуляторной цепи. Иммунология 1995; 3: 44-48.
- Иммунология. М.: Мир; 1987. 30-32.
- Пальцев М. А., Иванов А. А. Межклеточные взаимодействия. М.: Медицина; 1995. 39-59.
- Ярилин А. А. Система цитокинов и принципы ее функционирования в норме и патологии. Иммунология 1997; 5: 7-14.
- Джексенбаев О. Ш., Белобородое В. Б. ИЛ-1 и иммуностимуляция. Журн. микробиол. 1992; 7-8: 60-64.
- Буковская С. Н., Медуницын Н. В., Невская Л. В. и др. Динамика синтеза интерлейкина-1 р у больных и вакцинированных против вирусного гепатита В. Иммунология 1995; 6: 54-56.
- Волчкова Е. В., Алленов М. Н., Умбетова К. Т. и др. Сравнительная характеристика динамики регуляторных цитокинов у больных вирусными гепатитами А, В, С. Рос. Журн. гастроэнтерол., гепатол., колопроктол. 2001; 11 (1, Прил. № 12): 7.
- Ивашкин В. П. Клеточная и молекулярная биология воспаления печени. Там же 1998; 5: 13-17.
- Невская Л. В., Симбирцев А. С, Голбан Т. Д. и др. Интерлейкин-1 у здоровых и больных вирусным гепатитом В. В кн.: Материалы докладов Всероссийской науч.-практ. конф. "Состояние и перспективы разработки препаратов для диагностики вирусных гепатитов". Пермь; 1993. 89- 90.
- Носик Н. Н. Цитокины при вирусных инфекциях. Вирусология 2000; 9: 4-9.
Supplementary files
