Terapevticheskii arkhivTerapevticheskii arkhiv0040-36602309-5342LLC Obyedinennaya Redaktsiya3389910.26442/00403660.2020.03.000554Research ArticleChanges in gut microbiota with bronchial asthmaZolnikovaO. Yu.ks.med@mail.ruhttps://orcid.org/0000-0002-6701-789XPotskhverashviliN. D.ks.med@mail.ruKudryavtsevaA. V.ks.med@mail.ruKrasnovG. S.ks.med@mail.ruGuvatovaZ. G.ks.med@mail.ruTruhmanovA. S.ks.med@mail.ruKokinaN. I.ks.med@mail.ruIvashkinV. T.ks.med@mail.ruSechenov First Moscow State Medical University (Sechenov University)Engelhardt Institute of Molecular Biology27032020923566026042020Copyright © 2020, Consilium Medicum2020<p class="_4_resume ParaOverride-1" style="text-align: justify;"><span class="CharOverride-3"><strong>Aim.</strong></span>To study the intestinal microbiota changes in patients with bronchial asthma (BA).</p>
<p class="_4_resume ParaOverride-1" style="text-align: justify;"><strong><span class="CharOverride-3">Materials and methods.</span></strong>40 patients and 15 healthy individuals were included for the study. The microbiota study in feces samples was performed by sequencing the 16SpRNA gene.</p>
<p class="_4_resume ParaOverride-1" style="text-align: justify;"><strong><span class="CharOverride-3">Results.</span></strong>It was noted an increasing of the<span class="CharOverride-2">Proteobacteria</span>proportion in the patients with BA. The fractions of<span class="CharOverride-2">Betaproteobacteria</span>и<span class="CharOverride-2">Gammaproteobacteria</span>were increased in the patients with allergic BA and at the same time, only the<span class="CharOverride-2">Gammaproteobacteria</span>part was increased in patients with non-allergic form of BA. It was found an increase in<span class="CharOverride-2">Bacilli</span>and a decrease in the proportion bacteria forming butyrate (<span class="CharOverride-2">Anaerostipes, Faecalibacterium</span>) and acetate (<span class="CharOverride-2">Alistipes</span>), which was corresponded to a decrease in the proportion of strict anaerobic symbionts and an increase in the proportion of opportunistic facultative anaerobes. The relative bacteria amount was reduced for the<span class="CharOverride-2">Negativicutes Erysipelotrichia, Bacteroidia classes</span>, the<span class="CharOverride-2">Erysipelotrichaceae</span>,<span class="CharOverride-2">Pseudomonadaceae, Rhodospirillaceae, Bacillaceae families</span>and for the kinds of<span class="CharOverride-2">Barnesiella, Paraprevotella, Pyrolobus, Bifidobacterium, Pseudomonas, Coprobacter, Bacillus</span>in the allergic asthma patients with syndrome of intensive bacterial overgrowth (SIBO) cases. In the non-allergic asthma case, the presence of SIBO was accompanied by the relative bacteria amount increasing of the<span class="CharOverride-2">Bacteroidaceae</span>and the<span class="CharOverride-2">Paraprevotella</span>families and the<span class="CharOverride-2">Odoribacter</span>,<span class="CharOverride-2">Bacteroides, Butyricicoccus, Parasutterella</span>genera. The bacterial spectrum changes correlated with the main clinical and laboratory manifestations of BA in the patients.</p>
<p class="_4_resume ParaOverride-1" style="text-align: justify;"><span class="CharOverride-3"><strong>Conclusion.</strong></span>The results have indicated the differences in the intestinal microflora composition of healthy volunteers and patients with bronchial asthma in including the SIBO presence. It is necessary more detail study of the bacterial composition changes in the intestine for the bronchopulmonary pathology case.</p>intestinal microbiotabronchial asthmasyndrome of intensive bacterial overgrowth in the small intestineprobioticsкишечная микробиотабронхиальная астмасиндром избыточного бактериального роста в тонкой кишкепробиотики[Kåhrström C, Pariente N, Weiss U. Intestinal microbiota in health and disease. Nature. 2016;535(7610):47. doi: 10.1038/535047a][Kang YB. Gut microbiota and allergy/asthma: From pathogenesis to new therapeutic strategies. Allergol Immunopathol. 2016;3:799-804. doi: 10.1016/j.aller.2016.08.004][Ozdemir O. Microbial dysbiosis in allergic lower airway disease (asthma). MOJ Immunol. 2018;6(4):129-32. doi: 10.15406/moji. 2018.06.00207][Caricilli AM, Saad MJ. The role of gut microbiota on insulin resistance. Nutrients. 2013;5:829-51. doi: 10.3390/nu50308298][Mukhopadhya I, Hansen R, El-Omar EM. IBD-what role do Proteobacteria play? Nat Rev Gastroenterol Hepatol. 2012;9:219-30. doi: 10.1038/nrgastro.2012.14][Hevia A, Milani C, López P, Donado CD. Allergic Patients with Long-Term Asthma Display Low Levels of Bifidobacterium adolescentis. PLoS ONE. 2016;11(2):e0147809. doi: 10.1371/journal.pone.0147809][Chung K. Airway microbial dysbiosis in asthmatic patients: A target for prevention and treatment? Clin Rev Allergy Immunol. 2018;2:1071-81. doi: 10.1016/j.jaci.2017.02.004][Charlson ES, Bittinger K, Haas AR, et al. Topographical continuity of bacterial populations in the healthy human respiratory tract. Am J Respir Crit Care Med. 2011;184(8):957-63. doi: 10.1164/rccm.201104-0655OC][Huang YJ, Charlson ES, Collman RG, et al. The role of the lung microbiome in health and disease. A national heart, lung, and blood institute workshop report. Am J Respir Crit Care Med. 2013;187(12):1382-7. doi: 10.1164/rccm.201303-0488WS][Wang Q, Garrity GM, Tiedje JM, Cole JR. Naïve Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microbiol. 2007;73(16):5261-7. doi: 10.1128/AEM. 00062-07][Anand S, Mande SS. Diet, Microbiota and Gut-Lung Connection. Front Microbiol. 2018;9:2147. doi: 10.3389/fmicb.2018.02147][Zhang J, Guo Z, Xue Z, et al. A phylofunctional core of gut microbiota in healthy young Chinese cohorts across lifestyles, geography and ethnicities. ISME J. 2015;9(9):1979-90. doi: 10.1038/ismej.2015.11][Поцхверашвили Н.Д., Зольникова О.Ю., Кокина Н.И. и др. Синдром избыточного бактериального роста у больных бронхиальной астмой. Рос. журн. гастроэнтерологии, гепатологии и колопроктологии. 2018;28(4):47-54 [Potskhverashvili ND, Zolnikova ОYu, Kokina NI, et al. Small Bowel Bacterial Overgrowth Syndrome in Patients with Bronchial Asthma. Russian J Gastroenterol Hepatol Coloproctol. 2018;28(4):47-54 (In Russ.)]. doi: 10.22416/1382-4376-2018-28-4-47-54]